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Yeast Starter shelf life in fridge question

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Post time 2022-12-10 12:14:33 | Show all posts |Read mode
Hi All. My usual procedure is to create my starter, let it ferment for about 4-5 days at room temp, then cold crash it in the fridge overnight, pour off 80-90% of the liquid, then pitch the yeast. This works well for me.
My problem this time is that I'll be away until the day before I plan on making my next beer -- so I won't have time to make the starter when I get back. I'll be away for 7 days. I'm pretty sure I've read that a starter can last a few weeks in the fridge without degrading (much). So if I make it before I go, (timing it so that it finishes about when I need to leave), would it be fine in the fridge for those 7 days?
Thanks,
Dan
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Post time 2022-12-10 12:26:59 | Show all posts
Short answer: Yes you're fine.
Long answer: (from Chris White's book Yeast pg 160-161) Ideally use harvested or propagated yeast within 1-3 days. After 4 weeks yeast viability is 50% or less, so the yeast should be tossed (or re-propagated). Ideally you want yeast viability of 90% or greater, so consider 14 days the maximum storage time.
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Post time 2022-12-10 12:31:48 | Show all posts
MoreBeer has a viability calculator on their website, if you want to get specific. Amazon has 400x microscopes if you want to get really specific.
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Post time 2022-12-10 13:00:58 | Show all posts
If you can, overbuild your starter to compensate for the loss of cells during aging. You'll likely lose around 10-20%, so shoot for ~120% of the cells you'll need to pitch. Good luck!
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Post time 2022-12-11 08:17:18 | Show all posts
It will keep just fine for a week. Or even 2 weeks. Beyond that point, I might feed it more sugar to help wake it up again.
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Post time 2022-12-11 08:25:09 | Show all posts
REal world example of exactly this topic here. I made a two step starter, second step finished 11/16, but I did not brew with it until 12/5. The calculators said it would be about 85%, but I had aimed for a very high, 2.0m cells per degree Plato, pitch rate and figured 80% was still 1.79 pitch rate and fine.
It took 4 days for the exotherm to appear, where it usually takes a day or maybe two.

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 Author| Post time 2022-12-11 10:38:18 | Show all posts
Thanks, Everyone
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Post time 2022-12-11 17:50:03 | Show all posts
Do a vitality starter if you want the yeast to be in perfect condition, just pour off the supernatant and add some 10% w/v DME solution when you mash in. Even a few hours in new wort will allow it to hit the main wort up and running.
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Post time 2022-12-11 18:45:26 | Show all posts
On a stir plate, or just on the countertop, swirling a few times a day?
There's a huge difference in growth between those two "methods."
There's also the s-n-s (shaken-not-stirred) starter method. It's mostly used 24 hours (?), or thereabout, before pitching. That would be a good application in your case.
That's correct!
I've stored starter slurries for months, years even. But always make a fresh starter with all or some of it, depending on age and how much there is.
Here's the pitch/growth calculator I use:
Homebrew Dad's Online Yeast Starter CalculatorUse Homebrew Dad's Online Yeast Calculator to determine how many yeast cells you need to pitch into your wort.

www.brewunited.com

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Post time 2022-12-12 06:36:07 | Show all posts
Read Chris White's book and then read this article from Woodland Brewing.
Refrigeration Effects on Yeast Viability
Note that Woodland brewing is just doing a test on the yeast viability while Chris White runs a lab that sells yeast. You decide which is the one to believe.
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